Description: (William Seeds, MD)

AOD 9604 is a fragment of GH (growth hormone) polypeptide (amino acids 176-191)that has been shown to have lipid-reducing effects, similar to but more effective than GHon account of it not having adverse side effects of unmodified GH. AOD 9604 can regulate fat metabolism by stimulating lipolysis (the breakdown or destruction of fat) and inhibits lipogenesis (the transformation of nonfat food materials into body fat) both in laboratory testing; and in humans and animals. Recent studies also demonstrate that AOD 9604 possesses other regenerative properties associated with growth hormones. Currently, trials are underway to show the application of AOD 9604 in osteoarthritis, hypercholesterolemia, and bone and cartilage repair.

❖ Sequence: Tyr-Leu-Arg-Ile-Val-Gly-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe
❖ MW = 1815.1

❖ Stimulates bone differentiation and mineralization in adipose-derived mesenchymal stem cells (MSC)
❖ Promotes myoblast differentiation
❖ Promotes chondrocyte production of collagen and proteoglycan
❖ Stimulates stem cell differentiation toward bone, muscle, and cartilage repair

❖ Inhibits lipoprotein lipase activity in adipose tissue, stimulating lipolysis and adipocytes
❖ Tyrosine (TYR) in AOD maintains the stability of the amino acid sequence; this fragment holds the fat-reducing and tissue repair sequence and mimics the effect of HGH on lipid metabolism, without having growth-promoting or pro-diabetic effects.
❖ Unable to induce dimerization and thereby activation of the receptor (no competition with HGH).

Clinical Pearls:
❖ Consider adding AOD 9604 with the GLP-1s for patients that have 30 lbs or more of fat to lose or patient’s that are not getting the expected results with the GLP-1s alone. When doing this we suggest a minimum of 3 months in a row. We have found that trying AOD 9604 only for one month at a time does not yield the desired results.
❖ We recommend combining AOD 9604 with either a GLP-1 agonist or other peptides as we have not found it to yield consistent results (for weight loss) as a stand-alone treatment.
❖ A good adjunct when a patient is not a good candidate for any of the GHRPs (growth hormone releasing peptides); Although AOD 9604 is a fragment of the human growth hormone chain it will not promote growth hormone release.
❖ Communicate expectations with your patients: In layman’s terms, “ adding AOD9604 does not guarantee fast weight loss, but scientifically it is assisting with lipolysis (the breakdown of fat) and prevention of lipogenesis (the formation of new fat) helping your fat metabolism work more efficiently). Healthy weight loss is a journey, not a quick fix.

❖ Advitam dosing: 300 mcg SubQ 20 days on 10 days off
❖ 250 mcg BID for fat loss
❖ SubQ, Oral, topical
❖ Length of treatment varies; from 6 weeks to 3 months, up to 6 months
Possible side effects:
❖ No allergic Rxn
❖ GRAS in foods under the conditions of the intended use of AOD 9604